Advanced ASO Modifications & Conjugation Services

To overcome the challenges of nuclease degradation and off-target effects, chemical modifications are essential for functional Antisense Oligonucleotides (ASOs). At ACUBIOCHEM, we offer a wide range of state-of-the-art modifications to optimize the therapeutic potential, stability, and delivery of your ASO libraries.

Comprehensive Modification Options

We support various sugar, backbone, and nucleobase modifications to meet your specific research needs:

  • Sugar Modifications (2nd Generation):

    • 2'-MOE (2'-O-Methoxyethyl): Significantly increases nuclease resistance and binding affinity.

    • 2'-OMe (2'-O-Methyl): Enhances stability and reduces immune stimulation.

    • 2'-F (2'-Fluoro): Increases RNA binding affinity (Ideal for siRNA and ASO mixmers).

  • Conformationally Restricted Modifications (3rd Generation):

    • LNA (Locked Nucleic Acid): Provides the highest binding affinity toward complementary RNA targets, allowing for shorter ASO designs.

  • Backbone Modifications:

    • Phosphorothioate (PS) Linkage: Replacing the phosphodiester bond to drastically improve resistance to exonucleases and endonucleases.

  • Targeted Delivery Conjugations:

    • GalNAc Conjugation: The gold standard for liver-specific delivery, enhancing cellular uptake via ASGPR.

    • Lipid Conjugations: Including Cholesterol for improved cellular permeability.

Technical diagram showing ASO chemical modifications (2'-MOE, LNA, PS linkage) and GalNAc conjugation services by AcuBiochem.

Technical Specifications & Quality Assurance

FeatureSpecificationModification TypeSugar, Backbone, Linkers, and ConjugatesSynthesis Scale50 nmol to Gram levelsPurity>90% or >95% (determined by HPLC)Quality ControlFull MS and HPLC characterizationDelivery FormatLyophilized powder in tubes or 96-well plates

FEATURE

Specification

Modification Type

 Sugar, Backbone, Linkers, and Conjugates

Synthesis Scale

 50 nmol to gram levels

Purity

 >90% or >95% (determined by HPLC)

Quality Control

 Full MS and HPLC characterization

Delivery Format

 Lyophilized powder in tubes or 96-well plates

Why Choose AcuBiochem for ASO Modifications?

  • High Binding Affinity: Our LNA and 2'-MOE modifications ensure tight target binding.

  • Improved Pharmacokinetics: PS-linkages and terminal modifications extend in vivo half-life.

  • Customized Layouts: We provide flexible library formats for high-throughput screening.

Ready to Optimize Your ASO Library?

Our technical team is ready to assist you in selecting the best modification strategy for your target gene. Complete the form downbelow to reach out to our technicians for a fast evaluation.